Acquire your first fluorescence image
Draft outline
Scaffold. Replace the bullet prompts with your own text and delete this banner when done.
Learning-oriented, for users (biologists). If your FRAME is equipped with laser illumination, this tutorial captures a single fluorescence channel and then a multi-channel image.
Laser safety — read first
- Draft the safety rules for the installed lasers (405 / 488 / 520 / 635 nm): never look into the beam, interlocks, eyewear if applicable, who may operate.
TODO
Fill in the exact laser classes and the required safety wording for the FRAME.
Step 1 — Select a channel
- Which laser matches which dye (405 - DAPI, 488 - GFP, 635 - AlexaFluor 647, etc.).
- Turn off LED/brightfield, enable the fluorescence channel.
TODO image
Illumination panel with fluorescence channel selected. Notion / existing source:
tutorials/day-1/first-sample already shows the Illumination section with 488 nm.
Step 2 — Set exposure and power, avoid bleaching
- Balancing laser power vs. exposure; keeping power low to reduce photobleaching.
Step 3 — Multi-channel acquisition
- Sequentially acquire several channels; how they are combined/saved.
Step 4 — Save
- Channel metadata in OME-TIFF; opening in Fiji.