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Change the objective

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Learning-oriented, for users. The FRAME carries objectives on a motorized carriage; this tutorial covers switching between the mounted objectives in software and physically mounting a different objective.

Switch between mounted objectives (software)​

  • How to select the objective position in ImSwitch; what updates automatically (pixel size, NA, Z-offset).

TODO image

Objective selector in ImSwitch. Notion source: TASK-FR035 Show objective position in software, TASK-FR023 Highlighting current objective.

Physically mount a different objective​

  • RMS thread; parfocality / Z-offset consequences; when re-calibration is needed.
TODO

Cross-link the calibration that must follow an objective change: calibrate objective, pixel-size calibration. Notion source: TASK-FR029 Define and label Objective positions, TASK-FR030 Objective information in json file, TASK-FR026 Objective moves together with objective holder.